109 Limited genetic diversity of blaCMY-2-containing IncI1-pST12 plasmids from Enterobacteriaceae The current study is the first to explore blaCMY-2-containing IncI1–pST12 plasmids from related and unrelated isolates, using combined short- and long-read sequencing data. Moreover, this study includes isolates from different species, sequence types, and domains, both from human and broiler origin. Two different comparison techniques, either gene presence/absence and SNP differences, were used. Furthermore, combining long-read and short-read sequence data provided full plasmid analysis, including the presence of rearrangements. By combining the isolate collections from three different studies, we screened a relatively large amount of human and broiler cases. However, due to low prevalence of blaCMY-2 in the Netherlands, our sample size remained relatively small. This results in the main limitation of the current study, as the small sample size precludes the use of statistical test and caution must be applied, as the findings should be confirmed in a study with a larger sample size. Preferably, such a study should include isolates of different species, sequence types, and origin of isolation containing IncI1–pST12 plasmids. Furthermore, the current study only included plasmids of broilers isolated in one farm; therefore, other plasmids of veterinary origin should be added in future studies to confirm our findings. In conclusion, IncI1–pST12 plasmids of epidemiologically related and unrelated Enterobacteriaceae of both human and broiler origin in the current explorative study show a high degree of sequence similarity in terms of SNP differences and the number of shared genes. Supplementary Materials Supplementary materials can be found at https://www.mdpi.com/2076-2607/8/11/1755/ s1, Tables S1–S3, and Figure S1. Author Contributions Conceptualization, E.P.M.d.D., J.J.J.M.S., J.J.V. and J.A.J.W.K.; methodology, E.P.M.d.D. and J.J.J.M.S.; software, J.J.J.M.S.; formal analysis, E.P.M.d.D. and J.J.J.M.S.; investigation, E.P.M.d.D., J.J.J.M.S., C.V. and F.C.V.; resources, J.J.V., A.S., M.F.Q.K.- v.d.B. and J.A.J.W.K.; data curation, E.P.M.d.D. and J.J.J.M.S.; writing—original draft preparation, E.P.M.d.D. and J.J.J.M.S.; writing—review and editing, J.J.V., C.V., F.C.V., 6
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