151 In silico estimates of plasmid copy number and increased resistance in CMY-2-producing E. coli Table 1. Antimicrobial susceptibility and genetic characterisation of 20 ESBL-negative CMY2-positive E.coli isolates. E-test (mg/L) Isolate no. MLST CTX CAZ TZP ePCN CMY-2 number of acquired resistance encoding gene other than CMY-2 1 ST357 12 16 4 1.847 0 2 ST131 16 24 8 1.362 4 3 ST131 24 32 8 4.003 1 4 ST648 48 32 8 5.344 8 5 ST683 16 24 32 1.900 4 6 ST131 12 16 4 1.615 0 7 ST457 ≥256 ≥256 ≥256 9.941 0 8 ST5454 ≥256 64 ≥256 4.161 12 9 ST1196 ≥256 64 ≥256 4.266 11 10 ST38 ≥256 ≥256 ≥256 9.500 0 11 ST23 48 ≥256 ≥256 5.392 3 12 ST59 32 ≥256 ≥256 2.066 0 13 ST1722 ≥256 ≥256 ≥256 7.721 0 14 ST4118 8 24 8 2.399 4 15 ST1487 16 32 24 1.559 10 16 ST131 64 ≥256 ≥256 10.446 0 17 ST131 32 64 16 1.448 0 18 ST131 24 ≥256 ≥256 4.571 0 19 ST1485 16 24 8 1.870 6 20 ST345 ≥256 ≥256 ≥256 6.725 5 8
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