94 Chapter 5 Non-AmpC/non-ESBL producing E. coli from STEC-ID-net study In total 39 E. coli isolates obtained from stools of patients in a former multicentre prospective study, STEC-ID-net, performed from April 2013 to March 2014 in the Dutch regions of Groningen and Rotterdam were selected based on the absence of both AmpC, ESBL genes and known promoter/attenuator mutations related to AmpC hyperproduction (Ferdous M, Friedrich AW, Grundmann H, de Boer RF, Croughs PD, Islam MA, Kluytmans-van den Bergh MF, Kooistra-Smid AM 2016). Identification of resistance genes and MLST type and analysis of promoter/attenuator regions were performed as described above (pAmpC SOM study isolates) using the already available WGS data. Additional file 2 for this chapter is available online via: https://www.biorxiv.org/content/biorxiv/early/2019/09/30/787085/DC1/embed/media-1. pdf?download=true
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