136 CHAPTER 5 Figure 2. Benchmark of molecular profile preservation using K562 cancer cells. Profile preservation benchmarking using snap freezer vs liquid nitrogen. A. Unsupervised cluster analysis of all identified phosphosites of K562 suspension aliquots does not cluster samples frozen by liquid nitrogen separately from those frozen by the snap freezer, but clearly separates the +2hr positive control samples. Color key indicates Z-scores. B. UpSet plot indicating the number of overlapping phosphopeptides shared between (subsets of) the nine K562 samples. Fifteen out of 511 overlaps are shown, covering 51% of the data. C. Unsupervised cluster analysis of RNA expression of 100 most varying genes does not cluster samples frozen using liquid nitrogen or the snap freezer together, but separates the +2hr positive control samples. Color key indicates Z-scores.
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