Chapter 4 122 WL Mock WL+FR Mock WL NPA 50 μM WL+FR NPA 50 μM WL NPA 125 μM WL+FR NPA 125 μM 0 5 10 15 20 Pith layer number a a a a b a WL Mock WL+FR Mock WL NPA 50 μM WL+FR NPA 50 μM WL NPA 125 μM WL+FR NPA 125 μM 0 500 1000 1500 2000 2500 Pith thickness (μm) a c a a b b WL Mock WL+FR Mock WL NPA 50 μM WL+FR NPA 50 μM WL NPA 125 μM WL+FR NPA 125 μM 0 50 100 150 Interfascular cambium thickness (μm) a ab ab b b ab WL Mock WL+FR Mock WL NPA 50μM WL+FR NPA 50μM WL NPA 125 μM WL+FR NPA 125μM 0 20 40 60 Epidermis cell length (μm) a b ca a b b WL Mock WL+FR Mock WL NPA 50μM WL+FR NPA 50μM WL NPA 125 μM WL+FR NPA 125μM 0 50 100 150 Parenchyma cell length (μm) ab a ab b c c WL Mock WL+FR Mock WL NPA 50μM WL+FR NPA 50μM WL NPA 125 μM WL+FR NPA 125μM 0 50 100 150 200 250 Pith cell length (μm) c d a b a b WL Mock WL+FR Mock WL NPA 50μM WL+FR NPA 50μM WL NPA 125 μM WL+FR NPA 125μM 0 50 100 150 Collenchyma cell length (μm) a a a a b b (a) (b) (c) (d) (e) (f) (g) (h) Figure 4.9. Cell type responses to different NPA concentrations. We treated 14 day-old tomatoes with different concentration NPA by brushing onto internode 1 and analyzed the cell type responses by microscopy. Data includes measurement of (a) cell types illustration of internode 1, (b) pith layer numbers, (c) pith thickness, (d) interfascicular cambium thickness, (e) epidermis cell length, (f) collenchyma cell length, (g) parenchyma cell length, and (h) pith cell length. Data are represented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). The sample size for each treatment was n=60, and the experiment was repeated twice.
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