Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 125 WL Mock WL+FR BBo 100μM WL+FR PBZ 100μM WL+FR BZ 100μM WL+FR Mock 0 10 20 30 40 Internode 1 length (mm) a b a a b WL Mock WL+FR BBo 100μM WL+FR PBZ 100μM WL+FR BZ 100μM WL+FR Mock 0 1 2 3 4 5 Internode 1 diameter (mm) a b ab ab ab (a) (b) (c) Figure 4.11. Stem response to 100 μM BBo, PBZ, BZ. We drained the soil 4 days before FR treatment, and applied BBo, PBZ and BZ directly to the soil 2 days before FR treatment in FR conditions. Data include measurements of (a) illustration of how to apply the soil penetration, (b) Internode 1 length, (c) Internode 1 diameter. Data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There are 12 biological replicates, and the experiment was repeated twice. 4.2.7 Auxin concentration in the internode is not affected by FR treatment Considering the induction of auxin response in the transcriptome and the effect of exogenous IAA, we were curious whether the IAA concentration changes in response to FR enrichment. To investigate this, we employed liquid chromatography-tandem mass spectrometry to quantify the IAA concentration in internode 1 in response to 1-hour, 2-hour, and 6-hour whole-plant FR treatments. Additionally, we assessed the potential circadian influence by subjecting samples to a 1-hour FR treatment in both the morning and afternoon (Figure 4.12). However, we did not uncover definitive evidence of IAA concentration increasing in response to FR in bulk tissue of internode 1, indicating that either IAA is not regulated, or that we missed subtle changes in concentration and/or distribution of IAA.
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