Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 129 WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 10 20 30 Pith layer number ab c ab b a WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 1000 2000 3000 Pith thickness (µm) a b c b c WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 20 40 60 80 100 Cambium thickness (μm) a b c c ac WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 20 40 60 80 Epidermis cell length (µm) a b b a b WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 10 20 30 40 Collenchyma cell length (µm) a b c a c WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 50 100 150 Parenchyma cell length (µm) a b c a b WL Mock WL 100μM GA WL 100μM GA +IAA WL+FR Mock WL+FR 100μM GA+IAA 0 50 100 150 200 Pith cell length (µm) a b c d b (a) (b) (c) (d) (e) (f) (g) (h) Figure 4.15. Cell type responses to different GA+IAA treatments. We treated 14 day-old tomatoes with different concentration GA+IAA by brushing onto internode 1and analyzed the cell types response by microscopy. Data includes measurement of (a) cell type illustration of internode 1, (b) pith layer numbers, (c) pith thickness, (d) interfascicular cambium thickness, (e)epidermis cell length, (f) collenchyma cell length, (g) parenchyma cell length, and (h) pith cell length. Data are represented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). The sample size for each treatment was n=80 and the experiment were repeated twice.
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