Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 141 ► Figure 4.22. Gene expression in response to FR for selected TFs and hormone-related and -responsive genes. We analysed gene expression using qPCR to examine the effects of FR treatment on internode and pith tissues at three different time points: 1 hour, 2 hours, and 6 hours. The expression levels were obtained by comparing the response to WL and WL+FR conditions. The following genes were investigated: (a) GATA, (b) AUXIN-RESPONSIVE PROTEIN SAUR20, (c) AUXIN-RESPONSIVE PROTEIN SAUR, (d) SIPIN3, (e) CHALCONE SYNTHASE 2, (f) AUXINREGULATED IAA22, (g) GIBBERELLIN 20-OXIDASE-2, (h) HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT2, (i) bHLH050, (j) BASIC-LEUCINE ZIPPER (BZIP) TF, (k) HOMEOBOXLEUCINE ZIPPER PROTEIN, (l) TCP20, (m) DIVARICATA, (n) XYLOGLUCAN ENDOTRANSGLYCOSYLASE B1, (o) EXPANSIN 8. The experiment included 4 biological replicates, each consisting of 8-12 plants. Additionally, 3 technical replicates were performed to ensure the accuracy of the measurements.
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