Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 143 ► Figure 4.23. Gene expression in response to hormones for select TFs and hormone-related and -responsive genes. We profiled gene expression using qPCR of hormone treatment with 100 μM GA, IAA, BR or GA+IAA, BR+GA+IAA or FR light after 2 hours to investigate expression in internode and pith tissues. The following genes were examined: a) GA20ox, b) HY5, c) IAA19, d) SAUR, e) SIPIN3, f) CHALCONE SYNTHASE 2, g) IAA22, h) GIBBERELLIN 20-OXIDASE-2, i) BASIC-LEUCINE ZIPPER (BZIP) TF, j) TCP transcription factor 20, k)DIVARICATA, l) SIPIN9, m) EXPANSIN 8, n) YUC9. To ensure reliability, the experiment included 4 biological replicates, each consisting of 8-12 plants. Moreover, 3 technical replicates were performed to validate the accuracy of the measurements. Figure 4.24. Correlations of hormone- and FR-induced gene expression changes in comparison to WL control. Pearson’s correlation matrix between FR and each individual hormone or GA+IAA, BR+GA+IAA with gene expression date from Figure 4.23. Significant differences at p<0.05 among relatedness treatments after post hoc multiple comparison are shown separately for each treatment on the left and representation of the fitness with dot colored in blue for positive correlation, red for negative correlation.
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