Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 147 other emphasizes a level of functional interchangeability between these hormones in FR. This mutual compensation indicates the collaborative nature of IAA, GA and BR signaling pathways in regulating plant elongation under FR conditions. 4.3.4 Summary In conclusion, these findings underscore the complexity of hormonal interactions in regulating stem elongation during the shade avoidance response. While IAA contributes significantly to the gene expression pattern resembling far-red light treatment, GA and BR also play pivotal roles in modulating elongation. The intricate interplay between these hormones suggests a dynamic and finely tuned regulatory network that orchestrates plant growth in response to changing light conditions. These studies collectively highlight the complexity of hormone interactions, particularly auxin, gibberellins, and brassinosteroids, in various aspects of plant growth, including shade avoidance responses. This study’s findings provide insights specific to tomato plants and their shade avoidance response. Further investigation guided by these fundamental works could lead to a comprehensive understanding of hormonal regulation for agriculture, exploration of hormones/inhibitors/ combination that can be applied to specific traits (elongation of stem). 4.4 MATERIAL AND METHODS 4.4.1 Multiblast analysis We sourced Arabidopsis Gene Ontology annotations from TAIR, extracted a list of genes annotated as responsive to GA, IAA, and BR, and conducted a multi-blast on Solgenomics to annotate these genes with corresponding names from TAIR. Gene list are put as supplement: https://docs.google.com/spreadsheets/d/12oYbPxeVWsOKH36 vFiQIC37vfTyk9ezhI52F8LwkKcE/edit?usp=sharing Table 4.1. GO term used for Arabidopsis hormone responsive gene multiblast in Solgenomic.net. GO term annotation GO:0009733 response to auxin GO:0009739 response to gibberellin GO:0009735 response to cytokinin GO:1902347 response to strigolactone GO:0009741 response to brassinosteroid GO:0009723 response to ethylene GO:0009751 response to salicylic acid GO:0009753 response to jasmonic acid
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