Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 153 Internode06h Internode24h Internode30h Internode48h Pith30h Pith48h Solyc01g005230 Solyc01g005350 Solyc01g014320 Solyc07g064990 Solyc09g091530 Solyc09g091540 Solyc09g091550 Solyc12g014500 LogFC -2 -1 0 1 Internode06h Internode24h Internode30h Internode48h Pith30h Pith48h ARG2 Solyc01g091170 MTS1 Solyc01g105890 MTS2 Solyc01g105880 JA1 Solyc05g007180 PROSYSTEMINSolyc05g051750 TPK1B Solyc06g005500 OPR3 Solyc07g007870 LA3 Solyc08g076930 JA2Solyc12g013620 LogFC -2 -1 0 1 Internode 06h Internode 24h Internode 30h Internode 48h Pith 30h Pith 48h Solyc01g009230 Solyc01g068200 Solyc01g088170 Solyc01g095700 Solyc03g007310 Solyc03g095780 Solyc04g071590 Solyc04g071600 Solyc06g024310 Solyc06g050500 Solyc06g061180 Solyc08g065410 Solyc08g076960 Solyc08g082180 Solyc09g015380 Solyc10g076410 Solyc10g079210 Solyc10g085310 Solyc11g071600 Solyc11g071620 Solyc12g055990 Solyc12g095970 LogFC -2 -1 0 (a) (b) (c) Figure S4.2. FR response of SA, JA and ABA-related genes in tomato. Heatmap generated from RNA-seq data illustrates the log fold change in gene expression associated with (a) salicylic acid (SA), and (b) jasmonic acid (JA), (c)ABA. Upregulation is represented in red, while downregulation is depicted in blue. WL Mock Leaf1 WL IAA 3*100 μM Leaf1 WL Mock In1 WL IAA 3*100 μM In1 WL+FR Mock Leaf1 WL+FR IAA 3*100 μM Leaf1 WL+FR Mock In1 WL+FR IAA 3*100 μM In1 0 20 40 60 80 Stem length (mm) ab c b c a c ab c WL Mock Leaf1 WL IAA 3*100 μM Leaf1 WL Mock In1 WL IAA 3*100 μM In1 WL+FR Mock Leaf1 WL+FR IAA 3*100 μM Leaf1 WL+FR Mock In1 WL+FR IAA 3*100 μM In1 0 10 20 30 40 50 Hypocotyl length (mm) a ab b b c d d d WL Mock Leaf1 WL IAA 3*100 μM Leaf1 WL Mock In1 WL IAA 3*100 μM In1 WL+FR Mock Leaf1 WL+FR IAA 3*100 μM Leaf1 WL+FR Mock In1 WL+FR IAA 3*100 μM In1 0 1 2 3 4 Hypocotyl diameter (mm) a b c c bc ab ab ab (a) (b) (c) Figure S4.3. Stem responses to 100 μM IAA brushing treatments applied either on leaf 1 or internode 1. We treated 14 day-old tomatoes with 100 μM IAA by brushing onto leaf 1 and internode 1. Brushing was applied 3 times over one-week treatment period. The data include measurements of (a) stem length, (b) hypocotyl length, (c) hypocotyl diameter. The data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There were 12 biological replicates, and the experiment was repeated twice.
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