Chapter 4 156 0 50 100 150 0 50 100 150 NPA concentration(μmol/L) Stem length(mm) WL Mock WL+FR Mock WL NPA WL+FR NPA 0 50 100 150 0 20 40 60 80 100 NPA concentration(μmol/L) Hypocotyl length(mm) WL Mock WL+FR Mock WL NPA WL+FR NPA 0 50 100 150 0 1 2 3 4 NPA concentration(μmol/L) Hypocotyl diameter(mm) WL Mock WL+FR Mock WL NPA WL+FR NPA (a) (b) (c) Figure S4.6. Stem response to different NPA concentrations. We treated the first internode of 13 dag tomatoes with 25, 50, 125 μM NPA (or mock) in WL conditions one day prior to the start of the one week FR treatment. The data include measurements of (a)stem length, (b) hypocotyl length, (c) hypocotyl diameter. Data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There are 12 biological replicates, and the experiment was repeated twice. WL Mock WL+FR Mock WL BBo 25 μM WL+FR BBo 25 μM WL BBo 100 μM WL+FR BBo 100 μM WL BBo 200μM WL+FR BBo 200μM 0 20 40 60 80 100 Stem length(mm) a b a bc a c a ac WL Mock WL+FR Mock WL BBo 25 μM WL+FR BBo 25 μM WL BBo 100 μM WL+FR BBo 100 μM WL BBo 200μM WL+FR BBo 200μM 0 20 40 60 Hypocotyl length(mm) a b a b a b a b WL Mock WL+FR Mock WL BBo 25 μM WL+FR BBo 25 μM WL BBo 100 μM WL+FR BBo 100 μM WL BBo 200μM WL+FR BBo 200μM 0 1 2 3 Internode 1 diameter(mm) c bc ab bc bc a ab abc WL Mock WL+FR Mock WL BBo 25 μM WL+FR BBo 25 μM WL BBo 100 μM WL+FR BBo 100 μM WL BBo 200μM WL+FR BBo 200μM 0 10 20 30 40 Internode 1 length(mm) a b a bc a c a c WL Mock WL+FR Mock WL BBo 25 μM WL+FR BBo 25 μM WL BBo 100 μM WL+FR BBo 100 μM WL BBo 200μM WL+FR BBo 200μM 0 1 2 3 4 Hypocotyl diameter (mm) c b abc bc bc a abc abc (a) (b) (c) (d) (e) Figure S4.7. Stem response to different BBo concentrations by spray. We treated the first internode of 13 dag tomatoes with BBo (or mock) in WL conditions one day prior to the start of the one-week FR treatment. The data include measurements of The data includes measurements of (a) Stem length (b). Hypocotyl length (c) First internode length (d) Hypocotyl diamete©(e) First internode diameter. Data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with’Tukey’s post hoc test (P<0.05). There are 12 biological replicates, and the experiment was repeated twice.
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