Hormone interplay in the regulation far-red-responsive stem elongation in tomato 4 157 0 25501000 25 50100 0 50 100 150 GA concentration(μM) Stem length(mm) a a ab bc cd cd cd d W LWL+F R 0 25501000 25 50100 0 20 40 60 GA concentration(μM) Hypocotyl length(mm) abc a a a c bc c c WL WL+FR 0 25501000 25 50100 0 1 2 3 4 GA concentration(μM) Hypocotyl diameter (mm) ns WL WL+FR (a) (b) (c) Figure S4.8. Stem response to different GA concentrations. We brushed the first internode of 14 day-old tomatoes with different concentration GA (or mock) in WL or FR conditions over one-week FR treatment. Brushing was applied 3 times over one week treatment period. The data include measurements of (a)Stem length, (b) Hypocotyl length, (c) Hypocotyl diameter. (Data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There are 12 biological replicates, and the experiment was repeated twice. WL Mock WL+FR Mock WL+FR 25μM PBZ WL+FR 50μM PBZ 0 20 40 60 80 100 Stem length(mm) a b b b WL Mock WL+FR Mock WL+FR 25μM PBZ WL+FR 50μM PBZ 0 10 20 30 40 50 Hypocotyl length(mm) a ab b ab WL Mock WL+FR Mock WL+FR 25μM PBZ WL+FR 50μM PBZ 0 10 20 30 40 Internode 1 length(mm) a b b b WL Mock WL+FR Mock WL+FR 25μM PBZ WL+FR 50μM PBZ 0 1 2 3 4 5 Hypocotyl diameter (mm) ns WL Mock WL+FR Mock WL+FR 25μM PBZ WL+FR 50μM PBZ 0 1 2 3 4 Internode 1 diameter (mm) ns (a) (b) (c) (d) (e ) Figure S4.9. Stem response to different concentration PBZ. We brushed the first internode of 13 dag tomatoes with different concentration PBZ (or mock) in FR conditions one day in advance of one week FR treatment. Brushing was applied 3 times over one week treatment period. The data include measurements of (a) Stem length, (b) Hypocotyl length, (c) Hypocotyl diameter, (d) Hypocotyl diameter, (e) Internode 1 diameter. Data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There are 6 biological replicates.
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