Chapter 5 166 Brassica nigra (Black mustard) from Brassicaceae, and S. lycopersicum Moneymaker (Tomato), Capsicum annuum (Pepper), and Solanum melongena (eggplant) from the Solanaceae family, and Ocimum basilicum (Basil) from Lamiaceae family—to either white light (WL) or white light with supplemental far-red (WL+FR) conditions for one or two weeks, assessing the presence of the shade avoidance response. We found some conservation in the pith responses, but not across all dicots, and family-specific conservation of transcription factor response in SAS. This research can help us develop a further understanding of morphological and molecular regulation in SAS and how it has evolved across dicots. Figure 5.1 Species included in this study. This taxonomy common tree was generated by https:// www.ncbi.nlm.nih.gov/Taxonomy/CommonTree/wwwcmt.cgi. We also wanted to explore if our hypotheses could be addressed in the model species Arabidopsis thaliana Col-0 that has rosette growth habit instead. Arabidopsis has long served as a model system for exploring the shade avoidance response (SAS), so we explored the responses of the Arabidopsis inflorescence stem, which exhibits secondary growth and pith development. Using Arabidopsis for addressing questions related to SAS is advantageous due to the availability of mutants and the ease of conducting transgenics in this model plant. However, regrettably we found that the inflorescence stem proved to be suboptimal for this purpose. In this chapter, we also delved into the conservation of transcription factor (TFs) gene expression within the context of SAS across multiple plant species. For the analysis of
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