Exploring conservation of cellular-level traits in shade avoidance syndrome among species 5 181 ► Figure 5.8. Basil (O.basilicum) stem elongation is slow in response to FR. O. basilicum seedlings were subjected to two weeks of WL+FR treatment versus WL treatment. (a) Phenotype of O.basilicum in FR or WL. The phenotyping data include measurements of (b) stem length, (c) hypocotyl length, (d) internode1 length (e) hypocotyl diameter, and (f) internode1 diameter. The data are presented as mean ± SEM, and different letters indicate significant differences between treatments based on ANOVA analysis with Tukey’s post hoc test (P<0.05). There are 18 biological replicates, and the experiment was repeated twice. The following microscopy analysis results aspects are examined: (g) pith thickness from cross sections, (h) number of cell layers from cross section, (i) cell length from longitudinal sections, and (j) vasculature bundle area from cross section. Asterisks in the figure indicate the significance of differences between WL and WL+FR treatments, with the following significance levels: * p≤0.05; ** p≤0.01; *** p≤0.001. Error bars represent the standard error (SE), and the sample size for each measurement is n=40.
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