Exploring conservation of cellular-level traits in shade avoidance syndrome among species 5 203 ► Figure S5.3. Solyc08g080150 transcript abundance in response to FR treatment. Analysis was conducted using qPCR to examine the transcript abundance in internode 1 and its pith at two different time points: 6 hours, and 24 hours on top homologs in multiple species. The expression levels were obtained by comparing the response to WL and WL+FR conditions. The following genes were investigated: (a) Tomato: Solyc08g080150, (b) Pepper: TCP19, (c) Eggplant: chr8:85852001-85853900, (d) Arabidopsis1: AT2G45680, (e) Arabidopsis2: AT5G51910, (f) Mustard: BniB08g029800.2N.1, (g) Soybean1: Glyma.07G080300.1, (h) Soybean2: Glyma07g08710.2, (i) Pea1: XM_051030490.1, (j) Pea2: XM_051030491.1. The experiment included 4 biological replicates, each consisting of 8-12 plants. Additionally, 4 technical replicates were performed to ensure the accuracy of the measurements.
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