Cellular anatomy of tomato stems in response to far-red light 2 35 aspects of leaflets might have changed, such as pigment composition or cellular structure. We first followed up with fresh and dry weight measurements of leaf discs of fixed area. Both the fresh weight and the dry weight decreased in response to FR in MM, with a similar trend in M82 (Figure 2.8). This indicates that the plants may have formed leaf area with reduced investment in carbon, thus making relatively cheaper leaves. Cotyledon1 Cotyledon2 leaf1 leaf2 leaf3 leaf4 leaf5 leaf6 leaf7 0 20 40 60 80 WL+FR M82 WL M82 WL+FR MM WL MM Leaf order Leaf area(cm2) Figure 2.7. Leaf area of internode 1 of three-week-old M82 and Moneymaker after seven-day WL and FR+WL treatments. No significant differences were found by student’s t-test. Biological replicates n=18 and experiments were repeated for 3 times. Fresh weight Dry weight 0.000 0.002 0.004 0.006 0.008 0.010 WL M82 WL MM * * Weight(g) WL+FR M82 WL+FR MM ✱✱✱ ✱ Figure 2.8. Leaf disc weight of three-week-old M82 and Moneymaker after seven-day WL and FR+WL treatments. Asterisks indicate significance between WL and WL+FR as follows: * p≤ 0.05; ** p≤0.01, *** p≤0.001. Biological replicates n=6 and experiments were repeated for 3 times.
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