Cellular anatomy of tomato stems in response to far-red light 2 41 4D 7D 11D 0 50 100 150 Treatment Duration Cambium thickness(um) WL+FR WL 200 M82 4D 7D 11D 0 50 100 150 200 Treatment Duration Cambium thickness(um) WL+FR WL Moneymaker *** *** *** *** (a) (b) Figure 2.14. Cambium thickness of three-week-old (a) M82 and (b) Moneymaker in WL and FR+WL at different durations of treatment. Significance between WL and WL+FR is denoted by asterisks (* p≤0.05, ** p≤0.01). Error bars represent ± SE. There are 12 biological replicates, and the experiment was repeated twice. Figure 2.15. Internode 1 length against cambium thickness in (a) M82 and (b) Moneymaker. We combined phenotyping data (internode 1 length) and microscopy data (cambium thickness) from 4D, 7D and 10D FR treated 14-day-old tomatoes, and plotted the regression relationship with R. 2.2.6 Internode 1 has more pith layers and longer pith cells in FR than in WL We measured pith cell layer number, diameter, and area from cross sections (Figure 2.16). The pith cell lengths were calculated from longitudinal sections. Figure 2.8 showed that far-red enrichment induced larger diameter in the internode 1, which could be explained by the pith (Figure 2.16) and cambium increases. Furthermore, pith layers were increased in WL+FR, but only significantly in M82 and not in Moneymaker. The ratio of pith thickness to diameter in M82 increased by 7%.
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