Chapter 2 42 We further measured cell lengths for 60 cells per cell type in each biological replicate from five independent sets of internode samples (Figure 2.17). We found that pith cells located in the middle of the stem (“Pith-middle”) elongated significantly, and so did epidermis. To follow up, we used dental paste to make imprints of the epidermis, allowing us to measure cell lengths along internode 1 (Figure 2.18). More than 200 cells were analyzed in this method. We found that MM and M82 display differences: the MM basal epidermis cells (close to the cotyledons) were not significantly different between the treatments, while all the other comparisons showed significantly increased epidermis lengths in the WL+FR. Figure 2.16. Pith cell parameters of M82 and Moneymaker in WL and WL+FR. The data includes measurements of (a) pith thickness, (b) pith layer number, (c) pith radial ratio (pith thickness divided by stem diameter), (d) pith cell diameter, (e) pith cell length, and (f) pith cell area. Data are presented as mean ± SEM, Significance between WL and WL+FR is denoted by asterisks (* p≤ 0.05; ** p≤0.01, *** p≤0.001). Error bars represent ± SE. There are 18 biological replicates, and the experiment was repeated 3 times.
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