Cellular anatomy of tomato stems in response to far-red light 2 51 petiole, and scanned them with the Epson Perfection V800 Photo scanner using WinRhizo software (Regent Instruments Inc.) For each treatment, we used at least 6 replicates for each independent biological replicate, and this experiment was repeated for at least three times. 2.4.3.3 Leaf weight measurement To understand the changes in leaf anatomy, we measured the leaf weight. The leaf discs were taken as shown in Figure 2.22. We took 6 leaf discs from the third true leaf (avoiding main veins and the largest side veins). We measured the fresh weight of and the dry weight. They were taken from the third true leaf avoiding the main veins. The fresh weight was measured with digital scales, and then all the leaf discs were put into a drying oven at 30°C and dried for 4 days. We used the same digital scales with to measure the dry weight. Figure 2.22. Leaf disc sampling from the third leaf. 2.4.3.4 Chlorophyll content measurements The chlorophyll content was measured with, the living plants by FLIR A655sc highresolution science grade LWIR camera on the third leaf. The LWIR camera clip was clicked to the big leaflet of the third leaf for 3 seconds until the reading was obtained. We measured 8 plants for each treatment.
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