132 Chapter 6 DMEM-Glu/L 21% O2 24h 1% O2 4h 1% O2 8h 1% O2 16h 1% O2 24h Calcein AM EthD1 Figure 1 A B C iPSC-CM ACTN1 cTnT DAPI ACTN1 cTnT 40 50 60 70 80 90 100 Cells (%) E D F G 0.0 0.5 1.0 1.5 Ratio EthD1/Calcein AM 0h 4h 8h 16h 24h p=0,04 21% O2 0 20 40 60 80 100 Viable cells (%) p=0,1 Cell death (488 nm) 105 104 103 0 50K 100K FSC-A 24h 21% O2 150K 200K 250K 102 101 0 Live CMs 24h 1% O2 H 105 104 103 102 101 0 0 50K 100K FSC-A 150K 200K 250K Ki67 0 2 4 6 8 10 Cells (%) Live CMs Triton X-100 RPMI-Glu/L RPMI-L RPMI-Lac/L DMEM-Glu/L RPMI-Glu/L RPMI-L RPMI-Lac/L 1% O2
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