214 Chapter 8 Figure 3: Characterization of long-term cultured human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and identification of differentially expressed genes between PLN-R14del and control hiPSC-CMs. A) An overview illustrating the study design of comparing transcriptome between long-term cultured PLN-R14del and control hiPSC-CMs in three different media. B) Metabolic marker (PPARA), sarcomeric markers (alpha-actinin and troponin-T), mitochondrial markers (ATP5A and HSP60), and lipid droplets (Nile red) were examined in shortterm (20 days) and long-term (160 days) cultured PLN-R14del and control hiPSC-CMs using immunofluorescence staining. Nuclei were stained by DAPI (blue). Confocal images were taken at 63x magnification. C) Heatmap showing the mRNA expression levels of selected markers in sarcomeres, ion channels, and metabolism in both PLN-R14del and control hiPSC-CMs were shown from the proliferation stage to the maturation stage. D) Heatmap showing the mRNA expression levels of genes involved in oxidative phosphorylation (GO: 0006119 ) in control and PLN-R14del hiPSC-CMs cultured in three different media.
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