232 Chapter 8 SUPPLEMENTARY FIGURES Supplementary Figure 1 can be downloaded from: https://assets.researchsquare.com/files/ rs-1902254/v1/e49a3acc04361a0cb84717f0.png Supplementary Figure 1: A) Heatmap representing differentially acetylated regions between PLN-R14del and control group. B) Flowchart representing the detection and annotation of differentially acetylated regions in PLNR14del patients versus controls. C) Manhattan plot showing the differentially acetylated regions in PLN-R14del versus control hearts. Green: hyperacetylated regions; Red: hypoacetylated regions; Black: non-significant regions. Supplementary Figure 2 can be downloaded from: https://assets.researchsquare.com/files/ rs-1902254/v1/a3db1858b3158e47981958f1.png Supplementary Figure 2: A) Four differentially acetylated regions depicted using the UCSC Genome Browser. In each example, the region overlaps with the putative promoter (upstream region, 5’UTR, first exons in region tracks) and shows a significant difference between PLNR14del patients and controls (dot plots). Arrow beginning indicates gene transcription start site. AcS = acetylation 437 signal. ENCODE = publicly available ENCODE consortium data default display. H3K27ac = layered H3K27ac ChIPseq data in 6 cell types (GM12878 - red, H1-hESC – orange, HSMM – green, HUVEC – light blue, K562 – dark blue, NHEK – purple, NHLF – pink). DHS – DNaseI hypersensitivity clusters in 125 cells, TFs = ChIPseq for 161 TFs. B) Selected examples of enriched protein-protein interaction networks using protein-coding genes annotated to differentially annotated regions.
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