234 Chapter 8 Supplementary Figure 5 can be downloaded from: https://assets.researchsquare.com/files/ rs-1902254/v1/fcc80eaa09bc453ce53de50a.png Supplementary Figure 5: Genes annotated to PLN-specific differentially acetylated regions (ChIP-seq): As an effort to identify differentially acetylated regions in the vicinity of genes involved in lipid metabolism possibly linked to fibrofatty tissue replacement in PLN group, this figure shows selected examples of STRING protein database used to detect interacting proteins annotated to differentially annotated regions as part of the GO term ‘lipid metabolic process’ (GO:0006629). Only the highest confidence interactions are displayed. Disconnected nodes were removed from the network image. Dot plots represent the acetylation signal (AcS) measured for each sample. Supplementary Figure 6 can be downloaded from: https://assets.researchsquare.com/files/ rs-1902254/v1/c396eec02044df4930781f58.png Supplementary Figure 6: Differentially expressed genes between patients and controls (RNAseq): A) Top 5 enriched Pathway and GO: Biological process terms by upregulated genes in PLN versus control hearts. Notably, the most upregulated genes are part of fibrosis pathways and extracellular matrix organization. B) Top 5 enriched Pathway and GO: Biological process terms by downregulated genes in PLN versus control hearts. Note the enrichment of metabolic pathways. C) Transcription factor (TF) coding genes, which were predicted to bind to enriched motifs in H3K27ac ChIPseq differentially acetylated regions, with altered mRNA expression levels between PLN and control hearts.
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