270 Chapter 10 Figure 1. Generation and characterization of hiPSCs. (A) Typical round-shape colony morphology with small, tightly packed cells. (B) Expression of pluripotency-associated markers (OCT4, NANOG, and DAPI) by immunofluorescence. (C) The qRT-PCR analysis confirmed the expression of SOX2, MYC, NANOG, OCT4, and ZFP42. Total RNA from hiPSC-derived fibroblasts was used as negative control and reference. (D) Cardiac differentiation potential of hiPSC clones on day 11 (d11) post differentiation. (E) hiPSC-CMs show the capacity to expand up to passage 2 (P2). Scale bars represent 200 uM.
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