271 Generation of Human iPSC lines derived from PLN R14del Cardiomyopathy Patients 10 Table 3. Characterization and validation. Antibodies used for immunocytochemistry Antibody Dilution Company Cat # Pluripotency Markers Rabbit anti-NANOG 1:100 Cell Signaling, Cat# D73G4 Mouse anti-OCT3/4 1:200 Santa Cruz, Cat# sc-5279 Secondary antibody Goat anti-Rabbit IgG, Alexa Fluor 555 1:500 Thermo Fisher, Cat# A-21428 Primers Target Forward primer (5′-3′) Reverse primer (5′-3′) Pluripotency markers (qRT-PCR) Housekeeping marker SOX2 TGGACAGTTACGCGCACAT CGAGTAGGACATGCTGTAGGT MYC GCGAACCCAAGACCCAGGCCTGCTCC CAGGGGGTCTGCTCGCACCGTGATG NANOG TGCAAGAACTCTCCAACATCCT ATTGCTATTCTTCGGCCAGTT OCT4 AGGTGTTCAGCCAAACGACC TGATCGTTTGCCCTTCTGGC ZFP42 TAGAATGCGTCATAAGGGGTGA TCTTGCCTGTCATGTACTCAGAA RPL32 AGGCATTGACAACAGGGTTC GACGTTGTGGACCAGGAACT Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Acknowledgments This work was commissioned and completely supported by the Foundation PLN. A few additional chemicals were provided by NWO VENI grant (no. 016.176.136 to MH).
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