Renée Maas

293 Generation, high throughput screening, and biobanking of human iPSC-derived cardiac spheroids 11 REFERENCES 1. Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, et al. Chemically Defined and Small MoleculeBased Generation of Human Cardiomyocytes. Nat Methods. 2014;11: 855. 2. Kattman SJ, Witty AD, Gagliardi M, Dubois NC, Niapour M, Hotta A, et al. Stage-specific optimization of activin/ nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. Cell Stem Cell. 2011;8: 228–240. 3. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012;109. doi:10.1073/pnas.1200250109 4. Paige SL, Osugi T, Afanasiev OK, Pabon L, Reinecke H, Murry CE. Endogenous Wnt/beta-catenin signaling is required for cardiac differentiation in human embryonic stem cells. PLoS One. 2010;5. doi:10.1371/journal. pone.0011134 5. Gintant G, Burridge P, Gepstein L, Harding S, Herron T, Hong C, et al. Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Preclinical Cancer Drug Cardiotoxicity Testing: A Scientific Statement From the American Heart Association. Circ Res. 2019;125: e75–e92. 6. Ahmed RE, Anzai T, Chanthra N, Uosaki H. A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes. Front Cell Dev Biol. 2020;0. doi:10.3389/fcell.2020.00178 7. Liu C, Feng X, Li G, Gokulnath P, Xiao J. Generating 3D human cardiac constructs from pluripotent stem cells. EBioMedicine. 2022;76: 103813. 8. Musunuru K, Sheikh F, Gupta RM, Houser SR, Maher KO, Milan DJ, et al. Induced Pluripotent Stem Cells for Cardiovascular Disease Modeling and Precision Medicine: A Scientific Statement From the American Heart Association. Circ Genom Precis Med. 2018;11: e000043. 9. Buikema JW, Lee S, Goodyer WR, Maas RG, Chirikian O, Li G, et al. Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes. Cell Stem Cell. 2020;27: 50–63.e5. 10. Maas RGC, Lee S, Harakalova M, Snijders Blok CJB, Goodyer WR, Hjortnaes J, et al. Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc. 2021;2: 100334. 11. Tremblay C, Ruel J, Bourget JM, Laterreur V, Vallières K, Tondreau MY, et al. A new construction technique for tissue-engineered heart valves using the self-assembly method. Tissue Eng Part C Methods. 2014;20. doi:10.1089/ten.TEC.2013.0698 12. Lewis-Israeli YR, Wasserman AH, Gabalski MA, Volmert BD, Ming Y, Ball KA, et al. Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease. Nat Commun. 2021;12: 1–16. 13. Goldfracht I, Efraim Y, Shinnawi R, Kovalev E, Huber I, Gepstein A, et al. Engineered heart tissue models from hiPSC-derived cardiomyocytes and cardiac ECM for disease modeling and drug testing applications. Acta Biomater. 2019;92. doi:10.1016/j.actbio.2019.05.016 14. Fleischer S, Jahnke H-G, Fritsche E, Girard M, Robitzki AA. Comprehensive human stem cell differentiation in a 2D and 3D mode to cardiomyocytes for long-term cultivation and multiparametric monitoring on a multimodal microelectrode array setup. Biosensors and Bioelectronics. 2019. pp. 624–631. doi:10.1016/j.bios.2018.10.061 15. Branco MA, Cotovio JP, Rodrigues CAV, Vaz SH, Fernandes TG, Moreira LM, et al. Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture. Sci Rep. 2019;9: 9229. 16. Ergir E, La Cruz JO-D, Fernandes S, Cassani M, Niro F, Sousa D, et al. Generation and Maturation of Human iPSC-derived Cardiac Organoids in Long Term Culture. doi:10.1101/2022.03.07.483273 17. Lemoine MD, Mannhardt I, Breckwoldt K, Prondzynski M, Flenner F, Ulmer B, et al. Human iPSC-derived cardiomyocytes cultured in 3D engineered heart tissue show physiological upstroke velocity and sodium current density. Sci Rep. 2017;7: 5464. 18. Kofron CM, Kim TY, Munarin F, Soepriatna AH, Kant RJ, Mende U, et al. A predictive in vitro risk assessment platform for pro-arrhythmic toxicity using human 3D cardiac microtissues. Sci Rep. 2021;11: 1–16.

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