318 Chapter 12 Figure 7. Comparison of Ca2+ handling properties between cardiac hCSs from (isogenic) controls and PLNR14del patients. (A) Frames in time of spontaneous Ca2+ release in hCSs. Scale bar: 200 μm (B) Representative recording of time-lapse Ca2+ transients. (C) Measurement of Ca2+ handling parameters from automated recorded data. Comparison of (D) decay time (Tau), (E) Ca2+ transient amplitude, (F) Rise time, (G) transient duration 10% (CTD10), (H) transient duration 90% (CTD90), and (I) beating rate (BPM) among the combined groups of control lines ( biological replicates=2-3, n=188), controls + AAV.Ic-1 (biological replicates=2-3, n=151), PLN-R14del lines (biological replicates=2-3, n=243), and the PLN-R14del + AAV.Ic-1 (biological replicates=2-3, n=204) groups. * P< 0.05 - **** P < 0.0001 vs. each group by two-way ANOVA. Each dot represents the raw data of one individual spheroid without batch normalization.
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