333 Modeling and Rescue of PLN-R14del Cardiomyopathy Phenotype in Human iPSC-Derived Cardiac Spheroids 12 SUPPLEMENTARY FILES Supplementary Table 1: Antibodies and qPCR Primer sequences used in this study. Genes Sequence (5’-3’) Annealing Temperature (°C) CASQ2 Forward CAAACTGGAAGTCCAAGCCTT 55,7 - 63 Reverse GGCTCATCCATAAATGGCTCAT ATP2A2 Forward ACCCACATTCGAGTTGGAAG 57.1-65 Reverse CAGTGGGTTGTCATGAGTGG PLN Forward ACCTCACTCGCTCAGCTATAA 52.8-63.2 Reverse CATCACGATGATACAGATCAGCA MYH7 Forward TCTTTCCCTGCTGCTCTC 52.8-63.2 Reverse GACTGCCATCTCCGAATC ACTN1 Forward GACGAGAAGCTGGCTTGTATAG 60 Reverse CATAATACCTGAGCCTTGTCCC FN1 Forward AGCAGACCCAGCTTAGAGTT 60 Reverse GCAGAAGTGTTTGGGTGACT TNNI3 Forward CCAACTACCGCGCTTATGC 60 Reverse CTCGCTCCAGCTCTTGCTTT NPPA Forward CAACGCAGACCTGATGGATTT 60 Reverse AGCCCCCGCTTCTTCATTC Col1a1 Forward CTGCTGGACGTCCTGGTGAA 60 Reverse ACGCTGTCCAGCAATACCTTGAG GJA1 Forward TGGTAAGGTGAAAATGCGAGG 57.1-65 Reverse GCACTCAAGCTGAATCCATAGAT CASQ2 Forward CAAACTGGAAGTCCAAGCCTT 55,7 - 63 Reverse GGCTCATCCATAAATGGCTCAT PKP2 Forward GGTAGGAGAGGTTATGAAGAATGC 60 Reverse AAGCGATGAGAAGATGTGACG RPL32 Forward AGGCATTGACAACAGGGTTC 60 Reverse GACGTTGTGGACCAGGAACT aSMA Forward ACTGAGCGTGGCTATTCCTCCGTT 58 Reverse GCAGTGGCCATCTCATTTTCA Ki67 Forward GAAAGAGTGGCAACCTGCCTTC 60 Reverse GCACCAAGTTTTACTACATCTGCC
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