337 Modeling and Rescue of PLN-R14del Cardiomyopathy Phenotype in Human iPSC-Derived Cardiac Spheroids 12 SUPPLEMENTARY FIGURES Supplementary Figure 1 Supplementary Figure 1. Generation and characterization of hiPSCs. (A) Bright-field imaging revealed demarcated colony morphology of the newly generated lines 1CiCTR, 6BiR14del, and 10BiR14del. (B) Trilineage differentiation potential was shown with positive immunostainings of endodermal (SOX17), mesodermal (Brachyury T), and ectodermal (OTX2) markers in the newly generated lines 1CiCTR, 6BiR14del, and 10BiR14del. Scalebar = 400µm.(C) The qRT-PCR expression levels of NANOG and OCT4. Total RNA from human fetal cardiac fibroblasts (hfCF) was used as negative control and reference. (D) Expression of pluripotency-associated markers (OCT4, NANOG, and Hoechst) by immunofluorescence. (E) ddPCR summary of the detected copy number variations in the iPSC genome for karyotyping.
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