338 Chapter 12 Supplementary Figure 2 Supplementary Figure 2. Optimization of high throughput optical recordings of the Cal520 dye during the culturing of hCSs. (A) Representative Ca2+ transients and images of the optical recording over time in control hCSs (273iCTR line). (B) Representative Ca2+ transients and images of the optical recording over time in PLN-R14del hiPSC-derived CM spheroids (D4iR14del line). (C) Ca2+ transient parameters, including decay time, beating rate, duration at 10% decline from maximum amplitude (CTD10), and duration at 90% decline from maximum amplitude (CTD90). Each dot represents one individual spheroid and 3 biological (3 different differentiations of one hiPSC line) per condition were included in this analysis.
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