339 Modeling and Rescue of PLN-R14del Cardiomyopathy Phenotype in Human iPSC-Derived Cardiac Spheroids 12 Supplementary Figure 3 Supplementary Figure 3. Morphology, Purity, and Size of hCSs from healthy or isogenic controls (CTR; 1CiCTR, 273iCTR, C31iCTR) lines and PLN-R14del patients (R14del; D4iR14del, 6BiR14del, 10BiCTR) lines. (A) Bright-field images demonstrating the size of the hCSs after 21 days. Scale bar: 100 μm. (B) Flow cytometry quantification of alpha-actinin purity of each spheroid cell line before the generation of hCSs (Day 0). (C) Flow cytometry quantification of alpha-actinin purity of each spheroid cell line after 3 weeks of spheroid culturing (Day 21). (D) Quantification of spheroid size in the individual cell lines during culturing (Day 2, 7, 14, and 21). * P< 0.05 - **** P < 0.0001 vs. CTR group or each cell line by Student’s unpaired T-test or 2-way ANOVA. Each dot represents one individual spheroid and 2-3 biological replicates per condition were included in this analysis.
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