121 4 Docosahexaenoic acid prevents peroxisomal and mitochondrial protein loss in a murine hepatic organoid model Supplementary Figure 3. Effect of PPAR-α agonists on autophagic flux (A) GFP/RFP fluorescence intensities ratio in Huh7 cells in control conditions and 48 h of amino acid starvation treated and untreated with DMSO (vehicle) or WY-14643 (100 µM or 200 µM). Data represent mean ± SEM from 3 biological replicates (*P < 0.05, **P < 0.01, ****P<0.001 RM One-way ANOVA) (B) GFP/RFP fluorescence ratio in Huh7 cells in control conditions and 48 h of amino acid starvation treated and untreated with BSA (vehicle) or Linoleic acid (50 µM or 150 µM). Data represent mean ± SEM from 3 biological replicates (*P < 0.05, **P < 0.01 RM One-way ANOVA)
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