138 Chapter 5 presence of different acyl-CoAs (of different chain length) in the cell under different physiological conditions55. We did not find any absolute values for the Vmax of CRAT. Integrating kinetic data into a computational kinetic model of the peroxisomal β-oxidation To assess the physiological relevance of the kinetic parameters collected in studies with purified enzymes and peroxisomal fractions, we integrated the data into a computational model of the peroxisomal β-oxidation of straightchain acyl-CoAs. To predict the regulation of the peroxisomal β-oxidation flux and metabolite concentrations in mouse liver organoids under different physiological conditions, we adjusted the Vmax values based on proteomics obtained from these organoids. Building a computational model The computational model for the peroxisomal β-oxidation contains the enzymes depicted in Figure 1. Because of the limited kinetic information available, the first version of the model is restricted to oxidation of saturated straight-chain fatty acids containing an even number of carbons, starting from C18. It does not take transport into consideration yet.
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