139 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction Figure 1. Scheme of the enzymes included in the computational model. In yellow the enzymes that are part of the ‘traditional’ PPAR-inducible β-oxidation, which is modeled in this paper. In grey the enzymes not yet included in the model. Import and export are not considered in the model. Kinetic parameters implemented in the model were chosen from a selection of the papers cited above, based on a ranking according to their physiological relevance (Supplementary text 1). To obtain a complete parameter set, kinetic constants were combined from rat, mouse and human. Two substrate-two product reversible Michaelis-Menten kinetics (convenience kinetics56) were used as the starting point, unless stated differently. For example, the rate of ACOX1 was calculated according to Equation 1, which is based on the rate equation of ACADM previously published which takes into account substrate competition15. Equation 1. Rate equation for Acyl-CoA Oxidase 1. Rate equation is based on convenience kinetics56.
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