141 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction Table 1 shows the concentrations of the different metabolites at steady state. Metabolite Concentration (µM) C18 AcylCoA 150.0 C18 EnoylCoA 100.4 C18 KetoacylCoA 1.0 C16 AcylCoA 117.8 C16 AcylCarnitine 841.5 C16 EnoylCoA 9.2 C16 KetoacylCoA 0.1 C14 AcylCoA 107.1 C14 AcylCarnitine 765.0 C14 EnoylCoA 4.3 C14 KetoacylCoa 0.04 C12 AcylCoA 347.5 C12 AcylCarnitine 2481.8 C12 EnoylCoA 3.7 C12 KetoacylCoA 0.03 C10 AcylCoA 191.6 C10 AcylCarnitine 1368.8 C10 EnoylCoA 6.8 C10 KetoacylCoA 0.06 C8 AcylCoA 649.1 C8 AcylCarnitine 4636.5 C8 EnoylCoA 8.2 C8 KetoacylCoA 0.08 C6 AcylCoA 4.9 H2O2 9.5 Table 2 shows the fluxes through the different enzymes in the presence of the different chainlength substrates. Enzyme Flux (µmol·min-1·mgProt-1) ACOX1 for C18, C16, C14, C12, C10 and C8 1.6·10-4 DBP for C18, C16, C14, C12, C10 and C8 1.6·10-4 ACAA1 for C18, C16, C14, C12, C10 and C8 1.6·10-4 CROT for C16, C14, C12, C10 and C8 0 CRAT for C16, C14, C12, C10 and C8 0 CROT for C6 -6.4·10-6 CRAT for C6 -1.5·10-4 Catalase 9.6·10-4
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