155 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction is the reaction catalyzed by catalase, which is modelled as irreversible Michaelis-Menten, because of its extremely high equilibrium constant (Keq). In the case of CROT and CRAT, the reaction is described in the direction of the formation of acyl-CoA (reverse reaction), as the Vmax in the literature was measured in this direction. The rates of all the enzymes are given in µmol ·min-1 ·mg peroxisomal Protein-1. List of rate equations: ACOX1 For n= 8, 10, 12, 14, 16, 18: ( )) ( ( ) ( ) ( ) V sf Vmax − 1+ ∑ + · 1+ + · 1+ · 1+ ACOX ACOX ACOX C AcylCoa O K Acox C AcylCoa K Acox C EnoylCoA H O K Acox C AcylCoA K Acox K Acox n n C AcylCoA K Acox C AcylCoA C EnoylCoA K Acox C EnoylCoA H O K Acox H O O K Acox O CoA KAcox CoA C KetoacylCoA KAcox KetoacylCoA 1 1 · 1 · 1 · 1 · 1 · 1 = 8 = 18 1 1 1 1 1 1 C n C n n m n m O n m n m O eq n m n n m n m m i i 2 2 2 2 2 2 2 2 2 2 2 16 · · DBP For n= 8, 10, 12, 14, 16, 18: ( )) ( V sf Vmax − 1+ ∑ + · 1+ + DBPC DBP DBP C EnoylCoA NAD K DBP C EnoylCoA K DBP NAD C KetoacylCoA NADH K DBP C EnoylCoA K DBP NAD K DPB n n C EnoylCoA K DBP C EnoylCoA C KetoacylCoA K DBP C KetoacylCoA NAD K DBPNAD NADH K DBPNADH ·( ) · · · · = 8 = 18 ( ) n C n n m n m n m n m eq n m n n m n m m · · ACAA1 For n= 8, 10, 12, 14, 16, 18: ( ) ( ) ) ( ( ) V sf Vmax − 1+ ∑ + + · 1+ + ACAA C ACAA C ACAA C KetoacylCoA CoA K ACAA C KetoacylCoA K ACA CoA C AcylCoA NADH K ACAA C KetoaylCoA K ACAA CoA K ACAA n n C KetoacylCoA K ACAA C KetoacylCoA C AcylCoA K ACAA C AcylCoA AcetylCoA K ACAA AcetylCoA CoA K ACAA CoA NADHAcetylCoA K ACAA AcetylCoA 1 1 1 · 1 · 1 · 1 · 1 · 1 = 8 = 18 1 1 1 1 1 n n n m n m n m n m eq n m n n m n m m m −2 −2 −2 · · CROT = described in the direction of the Acyl-CoA (reverse reaction) For n = 6, 8, 10, 12, 14, 16 ( ) ( )) ( ( ) V sf Vmax − 1+ ∑ + · 1+ + CROT C CROT C CROT C AcylCarnitine CoA K CROT C AcylCarnitine K CROT C CoA C AcylCoA Carnitine K CROT C AcylCarnitine K CROTC CoA K CROT n n C AcylCarnitine K CROT C AcylCarnitine C AcylCoA K CROT C AcylCoA CoA K CROT C CoA Carnitine K CROT Carnitine · · · · · = 8 = 16 n n n m n m n n m n m n eq n m n n m n m n m · ·
RkJQdWJsaXNoZXIy MTk4NDMw