157 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction Kinetic parameters Parameter Value Value corrected Ref ACOX1 VmaxACOX1 3.130 µmol/min/ mg protein 0.04843 µmol/min/mg peroxisomal protein 2 sf_C18_ACOX1 0.65 / 2 sf_C16_ ACOX1 1 / 2 sf_C14_ ACOX1 1.1 / 2 sf_C12_ ACOX1 0.38 / 2 sf_C10_ ACOX1 1.06 / 2 Km_C18_AcylCoa_ ACOX1 9.6 µM 2 Km_C16_AcylCoa_ ACOX1 11.6 µM 2 Km_C10_AcylCoa_ ACOX1 20 µM 2 Km_C8_AcylCoa_ ACOX1 58.8 µM 2 Km_C18_EnoylCoa_ ACOX1 1.08 µM 3 Km_C16_EnoylCoa_ ACOX1 1.08 µM 3 Km_C14_EnoylCoa_ ACOX1 1.08 µM 3 Km_C12_EnoylCoa_ ACOX1 1.08 µM 3 Km_C10_EnoylCoa_ ACOX1 1.08 µM 3 Km_C8_EnoylCoa_ ACOX1 1.08 µM 3 Km_O2_ ACOX1 5 µM 3 Km_H2O2_ ACOX1 10 µM / Keq_ ACOX1 6.8·1012 / eQ Ki_Coa_ ACOX1 63.3 µM 4 Ki_C16ketoacyl-Coa 0.47 µM 2 DBP VmaxDBP 2.5 µmol/min/ mg protein 0.338 µmol/min/mg peroxisomal protein 5 sf_C18_DBP 0.44 / 6 sf_C16_DBP 0.82 / 6 sf_C14_DBP 1 / 6 sf_C12_DBP 0.96 / 6 sf_C10_DBP 0.87 / 6 sf_C8_DBP 0.62 / 6
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