159 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction Km_C14_AcylCoa_ ACAA1 13.83 µM 3 Km_C12_AcylCoa_ ACAA1 13.83 µM 3 Km_C10_AcylCoa_ ACAA1 13.83 µM 3 Km_C8_AcylCoa_ ACAA1 13.83 µM 3 Km_C16_AcylCoa_ ACAA1 13.83 µM 3 Km_AcetylCoa_ ACAA1 30 µM 3 Km_CoA_ ACAA1 16 µM 7 Keq ACAA1 2.49·105 EQ,7 CROT VmaxCROT 0.117 µmol/min/mg peroxisomal protein 8 sf_C18_CROT 0 / 9 sf_C16_CROT 0.25 / 9 sf_C14_CROT 0.51 / 9 sf_C12_CROT 0.125 / 9 sf_C10_CROT 0.62 / 9 sf_C8_CROT 0.85 / 9 Sf_C6_CROT 1 / 9 Km_C16_AcylCoa_CROT 5.2 µM 8 Km_C14_AcylCoa_CROT 5.3 µM 8 Km_C12_AcylCoa_CROT 8.3 µM 8 Km_C10_AcylCoa_CROT 10 µM 8 Km_C8_AcylCoa_CROT 6.5 µM 8 Km_C6_AcylCoa_CROT 23 µM 8 Km_C16_AcylCarnitine_CROT 13 µM 8 Km_C14_AcylCarnitine_CROT 4.6 µM 8 Km_C12_AcylCarnitine_CROT 5 µM 8 Km_C10_AcylCarnitine_CROT 20 µM 8 Km_C8_AcylCarnitine_CROT 48 µM 8 Km_C6_AcylCarnitine_CROT 220 µM 8 Km_carnitine C16_CROT 38 µM 8 Km_carnitine C14_CROT 64 µM 8 Km_carnitine C12_CROT 61 µM 8 Km_carnitine C10_CROT 68 µM 8
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