163 5 Establishing a peroxisomal β-oxidation computational kinetic model to understand the effects of amino-acid restriction Supplementary File 1. Supplementary Table 1. Protein concentrates and Vmax calculations. Supplementary File 1. Supplementary Table 1. Protein concentrates and Vmax calculations. Control Starvation + DMSO 100DHA concentration (fmol/ug protein) Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration (fmol/ug protCeainlc)ulated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) L009 ACOX1_Total 10.80764 0.04067 3.02388 0.01138 8.04155 0.03026 L011 10.41222 0.03918 2.87527 0.01082 17.78025 0.06691 L014 17.38712 0.06543 9.35665 0.03521 21.37775 0.08045 Vmax (µmol · min-1 · mg PeroxisomalProt-1) { E} Estimated Kcat Acox1 0.04843 12.86899 3763.30902 Control Starvation + DMSO 100DHA concentration (fmol/ug protein) Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration (fmol/ug protCeainlc)ulated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration (fmol/ug protein) Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) L009 CAT_Total 28.50415 7.83173 27.67596 7.60418 44.68941 12.27875 L011 41.79013 11.48215 24.02316 6.60054 33.38695 9.17332 L014 32.06871 8.81112 30.90474 8.49131 35.38155 9.72135 Vmax (µmol · min-1 · mg PeroxisomalProt-1) { E} Estimated Kcat Catalase 9.37500 34.12100 274757.51332 Control Starvation + DMSO 100DHA concentration (fmol/ug protein) Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration (fmol/ug protCeainlc)ulated vmax (µmol · min-1 · mg PeroxisomalProt-1) concentration (fmol/ug protein) Calculated vmax (µmol · min-1 · mg PeroxisomalProt-1) L009 CRoT_Total 5.77628 0.10392 5.82923 0.10487 8.43373 0.15173 L011 9.24114 0.16626 3.36615 0.06056 3.56954 0.06422 L014 4.49250 0.08082 4.56493 0.08213 5.88751 0.10592
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