172 Chapter 6 highlight that the differentiation protocol from iPSCs into EHOs recapitulates the early stages of hepatic development. Different functional assays as well as immunostainings were performed in the EHOs to determine their “liver-like” state. Immunostainings of the EHOs in culture revealed the expression of more mature hepatic markers ALB and HNF4-α (Figure 1f). Finally, the functionality of EHOs was confirmed by the secretion of albumin and urea into the supernatant (Figure 1g).
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