197 6 iPSC-derived liver organoids as a tool to study Medium Chain Acyl-CoA Dehydrogenase deficienc Supplementary Table 1. List of primer sequences used in RT-qPCR Gene Name Forward and reverse primer sequence (5’- 3’) SOX17 Fwd: CGCACGGAATTTGAACAGTA Rev: GGATCAGGGACCTGTCACAC FOXA2 Fwd: ACTACCCCGGCTACGGTTC Rev: AGGCCCGTTTTGTTCGTGA GATA4 Fwd: CCTCTTTCTCAGCAGAGCTGTA Rev: CTCTGCTACAGCCAGTAGGATT GATA6 Fwd: CCCACAACACAACCTACAGC Rev: GCGAGACTGACGCCTATGTA TBX3 Fwd: TTTCACAATTCTCGGTGGATGGTGGC Rev: ACGACTTTGGACATGCACTGTTCC HNF4α Fwd: ACTACATCAACGACCGCCAGT Rev: ATCTGCTCGATCATCTGCCAG Albumin Fwd: GAGACCAGAGGTTGATGTGATG Rev: AGTTCCGGGGCATAAAAGTAAG AFP Fwd: CTTTGGGCTGCTCGCTATGA Rev: GCATGTTGATTTAACAAGCTGCT CK19 Fwd: ACGACCATCCAGGACCTGCGG Rev: TCCCACTTGGCCCCTCAGCGTA SOX9 Fwd: ACTTGCACAACGCCGAG Rev: CTGGTACTTGTAATCCGGGTG ABCD1 Fwd: CCTTCTGGAACGCCTGTGGTAT Rev: TTCCAAGGCTGCCTTCTTCACG ABCD3 Fwd: GTTCCTTTAGCAACGCCAAATGG Rev: CTCTTTCCGCAGCCATTTGGAC NUDT7 Fwd: CTCCGTCCTTTTGCCATTGGTG Rev: TGTCTGTAGGGTCACGCTTACC NUDT19 Fwd: GCACCACTCGCCGCTTTGACA Rev: GTTGCCTCTGATGGAGATGACC SLC27A2 Fwd: GTGGAGAAAGATGAACCTGTCCG Rev: CTGAGCCTTTGCTCCAGCATAG ACBD2 Fwd: GCTGCCAGCAAGGATGACTCAA Rev: GCTTTCTCCTCTACTCCACCAG ACOT4 Fwd: CTTTGCCACGTTGGCTCTAGCT Rev: CCTAGAGAAATGCCCAAAAGCCC ACOT8 Fwd: GCTGACCACTGGATGCTCTATG Rev: AGGTCACAGCTAGGACTCCATC ACOX1 Fwd: GGCGCATACATGAAGGAGACCT Rev: AGGTGAAAGCCTTCAGTCCAGC EHHADH Fwd: CGGAGCATCGTGGAAAACAGCA Rev: CCGAGTCTACAGCAATCACAGG SCP2 Fwd: GACAAGGTGCAACGCTGGTTGA Rev: CCAGCAGAGTTCTGCACACTGA
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