58 Chapter 2 812968; the Canadian Institutes of Health Research (156307); and the Stichting De Cock-Hadders. SUPPLEMENTAL INFORMATION Supplemental information includes five figures and three tables. STATISTICAL ANALYSIS All results are expressed as mean ± standard error of the mean (SEM) as indicated. Biological replicates are considered as independent experiments. Group differences were assessed with repeated measures ANOVA for multiple day organoids size measurements or an ordinary one or two-way ANOVA with Tukey’s post hoc multiple comparisons analyses. Statistical analysis was performed with GraphPad Prism Software Version 9.02 (Graphpad Software, San Diego, California USA). Statistical significance was given as *** P value <0.001, ** P value < 0.01 and * P value < 0.5; NS or no indication means no significant changes. ACKNOWLEDGEMENTS We would like to thank Karen van Eunen, Niels Kloosterhuis and Marieke Smit for their technical support with intestinal organoids and animal work. We would also like to acknowledge Hans Clevers, Tomohiro Mizutani, Jeroen Korving and Klaas Nico Faber for their advice on culturing of organoids
RkJQdWJsaXNoZXIy MTk4NDMw