64 Chapter 2 SUPPLEMENTARY FILES Supplementary Figure 1. Differentiation of Murine Liver Organoids into Hepatocyte-like cells. Relative gene expression of stem cells markers (A) and early and mature hepatocyte markers (B) (C) measured in liver progenitor organoids and differentiated mature hepatic organoids as well as liver homogenate. Liver progenitor organoids expressed stem cell markers (e.g. LGR5, AXIN2) while differentiated mature hepatic organoids expressed early and mature hepatocyte markers (e.g. HNF4A, CYP1A2). Data represents 3 biological replicates from independent experiments. *p < 0.05, Mann-Whitney U test. (D) Representative immunoblot images (E)Immunoblot quantification of peroxisomal proteins ACOX1, Catalase and PGC1-α measured in the progenitors and differentiated organoids. Data represents 3 biological replicates from independent experiments. *p < 0.05, Unpaired t test. (F) Hepatocyte function test of liver organoids cultures in EM and DM shown as albumin secretion in the medium. Data represents 3 biological replicates from independent experiments. *p < 0.05, Paired t test.
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