65 2 Organoids as a model to study intestinal and liver dysfunction in severe malnutrition Supplementary Figure 2. Organoid size and growth rate of liver progenitor organoids cultured in control and aminoacid-free medium. (A) Representative images of liver progenitor organoids that were grown in complete culture medium (control) or amino-acid-free medium (starved) for 96 hours. Scale bar, 1000 μm. (B) Size of organoids in complete culture medium (control) or amino-acid free medium (starved), expressed as size of an organoid at every time point of interest. Data based on 3 sets of experiments in which 25 randomly selected organoids were tracked). Error bars indicate the SEM (*P<0.05, **P<0.01, *** P<0.001, unpaired t test at individual time points). Supplementary Figure 3. Characterization of murine intestinal organoids. Relative gene expression of stem cells and proliferation markers (A) and mature epithelial markers (B) measured in mouse jejunum and intestinal organoids. (A) Stem cell and proliferation markers include: LGR5, OLFM4 (olfactomedin 4), and Ki67. Mature epithelial markers include: CHGA (Chromogranin A) as a marker for enteroendocrine cells ; MUC2 (Mucin 2), as a marker for goblet cells ; VIL1 (Villin 1), as a marker for enterocytes and LYZ (Lysozyme) as a marker for Paneth cells.
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