66 Chapter 2 Supplementary Figure 4. Organoid size and number of crypts per organoid Organoids were grown in complete culture medium (control), amino-acid-free medium (starved), amino-acid-free medium for 48 h followed by complete culture medium for 48 h (re-supplementation). (A) The size of 26 organoids was measured every 24 hours for each experimental condition in 3 different organoid lines. (B) In addition, the number of crypts per organoid were assessed in the organoids that were measured in (A).
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