67 2 Organoids as a model to study intestinal and liver dysfunction in severe malnutrition Supplementary Figure 5. Relative gene expression markers for stem cells, proliferation and differentiation in intestinal organoids. Relative gene expression of stem cells markers (A), proliferation markers (B) and markers of differentiation (C) measured in intestinal organoids grown in complete culture medium (control), amino-acid-free medium (starved) or amino-acid-free medium for 48 h followed by complete culture medium for 48 h (re-supplementation). Data represents 3 biological replicates (independent experiments) (* P<0.05, ** P<0.01, Two-way ANOVA with Tukey’s post hoc test). AXIN2, axis inhibition protein 2, LGR5, Leucine Rich Repeat Containing G Protein-Coupled Receptor 5, OLFM4, olfactomedin 4 CHGA, chromogranin A; LYZ, lysozyme; MUC2, mucin-2; VIL1, villin 1.
RkJQdWJsaXNoZXIy MTk4NDMw