69 2 Organoids as a model to study intestinal and liver dysfunction in severe malnutrition Supplementary Table 2. List of primer sequences used in RT-qPCR Gene Name Forward and reverse primer sequence (5’- 3’) PPIA Fwd: TTCCTCCTTTCACAGAATTATTCCA Rev: CCGCCAGTGCCATTATGG LGR5 Fwd: GGAAATGCTTTGACACACATTC Rev: GGAAGTCATCAAGGTTATTATAA AXIN2 Fwd: TAAGCAGCCGTTCGCGATG Rev: TTCTTCCAGTTCCTCTCAG Ki67 Fwd: ATCATTGACCGCTCCTTTAGGT Rev: GCTCGCCTTGATGGTTCCT HNF4-a Fwd: GCTAAGGCGTGGGTAGGG Rev: AGGCTGTTGGATGAATTGAGG AFP Fwd: CCAGGAAGTCTGTTTCACAGAAG Rev: CAAAAGGCTCACACCAAAGAG CYP1a2 Fwd: TTCAGTCCCTCCTTACAGCC Rev: TCCAAGGCAGAATACGGTGAC CYP3a11 Fwd: TGGTCAAACGCCTCTCCTTGCTG Rev: ACTGGGCCAAAATCCCGCCG TBX3 Fwd: GAGGCCAAGGAACTTTGGGA Rev: AGGGAACATTCGCCTTCCTG Catalase Fwd: AGCGACCAGATGAAGCAGTG Rev: TCCGCTCTCTGTCAAAGTGTG ACOX1 Fwd: TAACTTCCTCACTCGAAGCCA Rev: AGTTCCATGACCCATCTCTGTC Albumin Fwd: GCGCAGATGACAGGGCGGAA Rev: GTGCCGTAGCATGCGGGAGG VIL1 Fwd: GCTTGCCACAACTTCCTAAGAT Rev: TCAGTTTAGTCATGGTGGACGA MUC2 Fwd: TGTGGAACCGGGAAGATG Rev: GACCACAGGTATGGTTCTGGA CHGRA Fwd: CGATCCAGAAAGATGATGGTC Rev: CGGAAGCCTCTGTCTTTCC OLFM4 Fwd: AGAAATCGTGGCTCTGAAG Rev: GTTCACCACACCACCATGAC LYZ1 Fwd: AGACCGAAGCACCGACTATG Rev: CGGTTTTGACATTGTGTTCG Mito Fwd: CCCAGCTACTACCATCATTCAAGT Rev: GATGGTTTGGGAGATTGGTTGATGT β-globulin Fwd: AAGGTGAACGCCGATGAAGT Rev: ATCAAAGTACCGCTGGGTCC Fwd, forward primer; Rev, reverse primer.
RkJQdWJsaXNoZXIy MTk4NDMw