115 4 Proteomic signature of macrophages cultured on collagen type 1 Figure 2: GO enrichment analysis of proteins upregulated by collagen type I. 69 proteins were >1.25-fold upregulated by macrophages cultured on collagen-coated plates (fibrous and globular pooled), compared to uncoated controls. a. PANTHER-Go Slim Biological Process analysis. b. PANTHER pathway analysis, indicating significant enrichment of the glycolysis pathway. c. Three proteins of the glycolysis pathway were significantly upregulated in macrophages grown on collagen (both types pooled, n=6 each) compared to macrophages grown on plastic (control, n=6). Protein intensity expressed as normalized Log2 values and statistically tested with an unpaired Wilcoxon test. p<0.025 was considered significant. Data represented as a box and whiskers plot, where the box extends from the 25th to 75th percentiles and whiskers from min to max values.
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