121 4 Proteomic signature of macrophages cultured on collagen type 1 Figure 5: Activity of key glycolytic enzymes in alveolar macrophages in different culture conditions. Macrophages were cultured in wells coated with either fibrous or globular collagen or uncoated. After 72 hours, enzymatic activity of four different glycolytic enzymes was measured in cell lysates. a-d. Activity of glycolytic enzymes compared between macrophages cultured in uncoated and collagen type I-coated conditions (fibrous and globular pooled). Only the activity of aldolase was significantly lower in collagen-coated conditions. e-h. Activity of glycolytic enzymes compared between macrophages cultured in fibrous collagen-coated and globular collagen-coated conditions. Only the activity of aldolase was significantly higher in globular collagencoated conditions. Bars represent the mean rate of enzyme activity and each data point represents a different biological replicate (n=4 for hexokinase, n=6 for phosphofructokinase, and n=8 for aldolase and phosphoglycerate kinase). Groups were compared using a Mann-Whitney U test (a-d) or Wilcoxon test (e-h). p<0.05 was considered significant. Data represented as a box and whiskers plot, where the box extends from the 25th to 75th percentiles and whiskers from min to max values.
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